Skip to main content
. 2010 Feb 24;84(9):4619–4629. doi: 10.1128/JVI.02406-09

FIG. 5.

FIG. 5.

PLP2 inhibits both RIG-I- and TLR3-dependent IFN-β induction. (A) HEK293 cells were transfected with IFN-β-Luc, pRL-TK, and either 300 ng of HCoV-NL63 PLP2 or 300 ng of SARS-CoV PLpro. At 24 h posttransfection, cells were either mock infected or infected with Sendai virus for 16 h. Following infection, cell lysates were prepared and assayed using the Dual-Luciferase reporter assay. (B) HEK293 cells were transfected with IFN-β-Luc, pRL-TK, 200 ng N-RIG, and either 300 ng of HCoV-NL63 PLP2 or 300 ng of SARS-CoV PLpro. At 24 h posttransfection, cell lysates were prepared and assayed using the Dual-Luciferase reporter assay. (C) HEK293-TLR3 cells were transfected with IFN-β-Luc, pRL-TK, and either 300 ng of HCoV-NL63 PLP2 or 300 ng of SARS-CoV PLpro. At 24 h posttransfection, cells were either mock treated or treated with poly(IC) for 6 h. Following poly(IC) treatment, cell lysates were prepared and assayed using the Dual-Luciferase reporter assay. Error bars indicate standard deviations from the means for triplicates.