FIG. 1.

Replication of HTCV-UC6 in an LLC-MK2 cell line. (A) Growth kinetics of HTCV-UC6 (stool filtrate) over 10 days with the addition of an anti-IFN-α antibody (triangles), an anti-TNF-α antibody (circles), both antibodies (diamonds), or no antibody (squares) to the culture medium. Viral titers are calculated as viral genome equivalents/ml. (B) A moderate cytopathic effect (CPE) was first visible at day 7 after infection with HTCV-UC6 and after addition of anti-IFN-α/anti-TNF-α antibodies to the culture medium. After infection with HTCV-UC6, a similar CPE was observed with the addition of the anti-IFN-α or anti-TNF-α antibody alone (data not shown).