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ICP0 induces colocalizing, conjugated ubiquitin in all VP22 mutant virus infections. Vero cells on coverslips were either uninfected (mock) or infected with the GFP-expressing viruses described in the legend for Fig. 4A at a multiplicity of infection of 10 and fixed with 4% paraformaldehyde 6 h later. Immunofluorescence was carried out for conjugated ubiquitin using antibody FK2 (red).
