TABLE 1.
Binding and assembly properties of VP22 truncation mutantsf
| VP22 residues | gE bindinga | VP16 co-IPb | ICP0 co-IPc | Virion VP22d | Virion ICP0e |
|---|---|---|---|---|---|
| 1-301 | + | + | + | + | + |
| 108-301 | + | + | −/+ | + | −/+ |
| 160-301 | + | + | − | + | − |
| 194-301 | − | − | − | −/+ | − |
| 1-212 | − | + | + | − | − |
| 1-165 | − | − | − | − | − |
a
Binding of VP22 truncation mutants to the cytoplasmic tail of gE as described previously (45).
b
Infected cell coimmunoprecipitation of VP16 with VP22 mutants as shown previously (22).
c
Infected cell coimmunoprecipitation of ICP0 with VP22 mutants as shown here.
d
Virion assembly of VP22 truncation mutants as described previously (22).
e
Virion assembly of ICP0 in viruses expressing VP22 truncation mutants as described here.
f
gE and VP16 assembly is unaffected by the absence of VP22. co-IP, coimmunoprecipitation.