TABLE 1.
Oligonucleotides used in this study
| No. | Name | Sequence (5′-3′) |
|---|---|---|
| 1 | NCRRz1-Fwd | CCTTAATACGAACTCACTATAGGAAAAAAAAAAAAAAACTGATGAGGCCGAAAGGCCG |
| 2 | NCRRz1-Rev | TTCGGCCTCATCAGTTTTTTTTTTTTTTTCCTATAGTGAGTCGTATTAAGG |
| 3 | NCRRz2-Fwd | AAAACCCGGTATCCCGGGTTCTTTTTTTTTTTTTTTCCTCCGAATTAAAGAAAAATTT |
| 4 | NCRRz2-Rev | TTCTTTAATTCGGAGGAAAAAAAAAAAAAAAGAACCCGGGATACCGGGTTTTCGGCCT |
| 5 | NCRRz3-Fwd | ACCCCTACAACAGTATGACCCAATCCAATTCGACTGAGGTAGGGTTACTA |
| 6 | NCRRz3-Rev | GATCTAGTAACCCTACCTCAGTCGAATTGGATTGGGTCATACTGTTGTAGGGGTAAATTT |
| 7 | NdeIC1pET22b | GGGGCATATGGCCAGCAACGTTACCAACAAG |
| 8 | EcoRIC1pET22b | GGGGGAATTCGGTCCTCCATTGGCGCTGTCTCT |
| 9 | CIDdisruptL187Q | CCTTCAGGCCATTAAGAAGGGGGAGACCCAGATAAAACAAAAAGTGG |
| 10 | CIDdisruptL187Q-r | CCACTTTTTGTTTTATCTGGGTCTCCCCCTTCTTAATGGCCTGAAGG |
| 11 | hcshRNAoligosense | GATCCCCCGTCAGCGTGTATCAGGAATTCAAGAGATTCCTGATACACGCTGACGTTTTTA |
| 12 | hcshRNAoligoanti | AGCTTAAAAACGTCAGCGTGTATCAGGAATCTCTTGAATTCCTGATACACGCTGACGGGG |
| 13 | hcshRNAScramfwd | GATCCCCCCGTAAGATCTGGGGCCTCCAAATACCTGTGGTGGAATAAATTCCAGTTTTTA |
| 14 | hcshRNAScramrev | AGCTTAAAAACTGGAATTTATTCCACCACAGGTATTTGGAGGCCCCAGATCTTACGGGGG |
| 15 | PV+Rev | CACTGTCCTGCTCTGGTTGG |
| 16 | PV-Rev | ACTCCTGACAACAACCAGACATC |
| 17 | β-actinRev | GGAGAAGCTGTGCTACGTCG |
| 18 | PV+Fwd | ATGTTCCTGTCGGTGCTGTG |
| 19 | PV-Fwd | GCGGGAACACAAAGGCATTC |
| 20 | β-actinFwd | GTGGATCAGCAAGCAGGAG |
| 21 | Linker 1F | CCGGAGGAAAAAAAAAAAAAAAA |
| 22 | Linker 1R | TTTTTTTTTTTTTTTTTTTTCCT |
| 23 | Linker 2F | AAAAAAAAACTATAGTGTCACCTAAATG |
| 24 | Linker 2R | AATTCATTTAGGTGACACTATAGTTTTT |
| 25 | HincIIBbsIF | TTAAAACAGCTCTGGGGTTGTACCCACCCCAGAGGCCCAC |
| 26 | HincIIBbsIR | CCCAGTGGGCCTCTGGGGTGGGTACAACCCCAGAGCTGTTTTAA |
| 27 | BspeIMut+ | GGGTCATACTGTTGTAGGGGTAAATTTTTCTTTAATCCGGAGGA |
| 28 | BspeIMut− | TCCTCCGGATTAAAGAAAAATTTACCCCTACAACAGTATGACCC |
| 29 | BspeIMutBck+ | GGGTCATACTGTTGTAGGGGTAAATTTTTCTTTAATTCGGAGGA |
| 30 | BspeIMutBck− | TCCTCCGAATTAAAGAAAAATTTACCCCTACAACAGTATGACCC |