TABLE 2.
Antiviral activities in influenza A/H1N1, A/H3N2, and B virus-infected MDCK cellsa
| Strain | Antiviral EC50b (μM) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 4c |
4d |
6b |
Ribavirin |
Oseltamivir carboxylate |
||||||
| CPE | MTS | CPE | MTS | CPE | MTS | CPE | MTS | CPE | MTS | |
| A/H1N1 | ||||||||||
| A/FM/1/47 | >50 | >50 | >50 | >50 | >50 | 38 ± 10 | 12 ± 3 | 13 ± 1 | 4.0 ± 2.9 | 4.0 ± 2.2 |
| A/Ned/378/05 | >50 | >50 | >50 | >50 | >50 | 21 ± 14 | 8.4 ± 2.2 | 6.1 ± 2.9 | 0.19 ± 0.06 | <0.20 |
| A/PR/8/34 | >50 | >50 | >50 | >50 | >50 | >50 | 10 ± 0 | 12 ± 5 | 12 ± 7 | 6.3 |
| A/H3N2 | ||||||||||
| A/X-31 | 3.3 ± 1.3 | 3.2 ± 2.0 | 2.4 ± 2.1 | 4.0 ± 3.8 | 2.6 ± 2.3 | 5.9 ± 4.3 | 9.9 ± 3.3 | 11 ± 4 | 0.0042 ± 0.0035 | 0.020 ± 0.040 |
| A/HK/2/68 | 7.4 ± 2.8 | 4.6 ± 3.0 | 3.4 ± 1.3 | 1.8 ± 1.4 | 5.4 ± 3.2 | 2.7 ± 1.1 | 11 ± 1 | 9.1 ± 1.3 | 1.2 ± 1.0 | <0.20 |
| A/Victoria/3/75 | 23 ± 17 | 27 ± 20 | 6.0 ± 1.5 | 3.7 ± 3.2 | 7.1 ± 3.9 | 7.2 ± 5.2 | 8.7 ± 3.7 | 7.7 ± 4.1 | <0.20 | <0.20 |
| A/HK/7/87 | 5.0 ± 2.3 | 3.2 ± 2.3 | 4.2 ± 3.3 | 4.3 ± 3.0 | 2.8 ± 1.8 | 1.4 ± 0.6 | 7.4 ± 2.1 | 7.2 ± 3.8 | 1.7 ± 1.9 | 0.63 ± 0.23 |
| A/Aichi/2/68 | 7.5 ± 2.7c | |||||||||
| A/H5N1 | ||||||||||
| A/Vietnam/1203/04 | >100c | |||||||||
| A/chicken/Vietnam/ C58/04 | >100c | |||||||||
| A/H7N2 | ||||||||||
| A/chicken/Pennsylvania/ 143586/02 | >100c | |||||||||
| B | ||||||||||
| B/HK/5/72 | >50 | >50 | >50 | >50 | >50 | >50 | 5.8 ± 2.9 | 11 ± 9 | 1.7 ± 0.9 | 2.2 ± 0.9 |
| B/Lee/40 | >50 | >50 | >50 | >50 | >50 | >50 | 15 ± 6 | 15 ± 9 | >20 | >20 |
| B/Ned/537/05 | >50 | >50 | >50 | >50 | >50 | >50 | 6.6 ± 3.1 | 7.9 ± 0.3 | 2.2 ± 2.3 | 1.7 ± 1.7 |
a
The data shown are the means ± standard deviations of 2 to 7 independent tests.
b
Antiviral activity is expressed as the EC50, defined as the compound concentration producing 50% inhibition of virus replication, as estimated by microscopic scoring of the CPE or by measuring cell viability in the formazan-based MTS assay.
c
Data obtained by plaque reduction assay.