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. 2010 Feb 10;84(9):4321–4329. doi: 10.1128/JVI.02280-09

FIG. 3.

FIG. 3.

DNMT3a can downregulate viral RNA and pregenomic RNA production. All data are shown as means ± standard errors of means. (A) After cotransfections, the levels of HBV cccDNA did not differ between the various conditions (all P values not significant). (B) Pregenomic RNA levels were decreased under all conditions compared to the level of the control of HBV plus empty vector (all P < 0.003). Active DNTM3a reduced pregenomic RNA levels more than methylated DNMT3a vectors (P < 0.001). (C) Precore RNA levels were decreased under all conditions compared to the level of the control of HBV plus empty vector (all P < 0.003). Active DNTM3a did not reduce pregenomic RNA levels more than methylated DNMT3a vectors (P = 0.9). However, fully methylated HBV did have lower precore levels than both active DNMT3a (P < 0.001) and methylated DNMT3a (P = 0.04) cotransfections.