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Jxx recombinants and their viability in Huh7.5 cell results from two independent experiments (Jxx_A and Jxx_B). (A) Genome map of cDNA clones (pJ6CF, gray; pJFH1, white). The NS3 protease and NS4A were replaced with intra- and intergenotypic genes (replaced region, black). (B to G) RNA transcripts from J1a1a, J1b1b, J2a2a, J3a3a, J4a4a-19, J5a5a, and J6a6a were electroporated into Huh7.5 cells. Jc1 and pJFH1-GND served as positive and negative controls, respectively.
