FIG. 8.

Antiviral inhibition of Jxxs. Reduction in viral replication. RNA (1 to 10 μg) was electroporated into Huh7.5 cells and incubated for 24 h. Cells were then washed and incubated in media containing 0.1% DMSO, as a carrier control, with or without the indicated doses of BILN 2061 for a further 72 h. The percent inhibition of replication was determined at 96 h postelectroporation (mean ± SEM; n = 3) and calculated as the ratio of NS5A-positive cells in BILN 2061-treated cells to those of the control without antiviral.