FIG. 9.

Decreasing lamin A/C greatly enhances production of the PKmut virus in 293 cells. (A) 293 cells infected with WT or PKmut EBV were treated twice with control siRNA (Si-C), or siRNA directed against the lamin A/C proteins (Si-lamin) prior to transfecting the cells with BZLF1/BRLF1/gp110 to induce lytic infection. The virus titer for each condition is shown (normalized to 1 for the amount of virus induced in the presence of the control siRNA). (B) Western blot analysis of total lamin A/C, BZLF1, and β-actin in control siRNA- and lamin A/C siRNA-treated cells.