TABLE 5.
Summary of results obtained with mutant proteins
| Mutant protein | Complementation of UL25 null mutanta | Full-length DNA packagingb | Virions in cytoplasmc |
|---|---|---|---|
| wt pUL25 | + | + | + |
| None | − | − | − |
| pUL25-C3A | + | ND | − |
| pUL25-C3B | − | − | − |
| pUL25-C4 | − | + | − |
| pUL25-L1 | + | ND | − |
| pUL25-L2 | + | ND | − |
| pUL25-L3 | − | + | − |
| pUL25-L4 | + | ND | − |
| pUL25-L5 | − | − | − |
| pUL25-L6 | − | + | + |
a
Complementation of growth of ΔUL25MO in Vero cells.
b
The ability to package full-length virus DNA in U2OS cells coinfected with ΔUL25MO and the recombinant baculovirus expressing the mutant protein. ND, not determined.
c
Detection of virions in the cytoplasm in U2OS cells coinfected with ΔUL25MO and the recombinant baculovirus expressing the mutant protein, based on electron microscopic and fluorescence in situ hybridization results.