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. 2010 Feb 17;84(9):4124–4135. doi: 10.1128/JVI.02319-09

FIG. 1.

FIG. 1.

BiFC analysis shows that ICP27 undergoes a head-to-tail intramolecular interaction. RSF cells were transfected with plasmid DNA from ICP27-Venus fusion constructs N-Venus/ICP27/C-Venus, N-Venus/ICP27 (L8A, L11A, L15A)/C-Venus, N-Venus/ICP27 (C483S, C488S)/C-Venus, and N-Venus/ICP27 (S16A, S18A) or were cotransfected with N-Venus-ICP27 and ICP27-C-Venus or with ICP27-N-Venus and ICP27-C-Venus as indicated. Eighteen hours after transfection, cells were infected with 27-LacZ at an MOI of 10. Venus fluorescence was visualized directly at 4 and 8 h after infection.