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. 2010 Mar 10;84(10):5212–5221. doi: 10.1128/JVI.00078-10

FIG. 2.

FIG. 2.

p63 protein levels are inversely correlated with miR-203 levels upon keratinocyte differentiation. (A) Total p63 protein levels in NHKs and NHK-31 cells upon differentiation. (B) Western blot analysis for total p63, ΔNp63α, p53, and involucrin in NHKs and keratinocytes stably expressing HPV-31 E6/E7 and E7. Levels in the undifferentiated state and following differentiation in methylcellulose for 48 h are shown. (C) Total p63 and p53 protein levels in undifferentiated and 48-h-differentiated NHKs and keratinocytes stably expressing HPV-16 E6/E7, E7, and E6. (D) Relative changes in miR-203 RNA and p63 protein levels in HPV-positive and -negative keratinocytes upon differentiation in methylcellulose for 48 h. miR-203 levels were normalized to NHK-E7 cell levels (set to 1) and p63 levels to those seen in NHKs. The results are from 4 independent experiments ± standard errors of the mean.