FIG. 1.

Schematic drawing of wild-type (WT), lef-2 knockout (lef2 KO), and repair bacmids used in this study. The lef-2 gene sequence was replaced by the CAT gene flanked by 50-nucleotide sequences homologous to the 5′- and 3′-end sequences of the lef-2 gene. Reporter cassettes containing the egfp gene under the control of the heat shock 70 promoter were inserted into the polyhedrin locus by site-specific transposition in E. coli to yield a bacmid backbone for further modifications. The first bacmid, bAc^wt-hE, contains a wild-type bacmid backbone with the insertion of egfp; in the second bacmid, bAc^Δlef2-hE, lef-2 was deleted; in the third bacmid, bAc^{Δlef2-Rep-hE}, a lef-2 expression cassette was reinserted for repair; in the fourth bacmid, bAc^{hE-lef2HA-polh}, the HA tag sequence was inserted at the 5′ end of lef-2, followed by a complete polyhedrin expression cassette. p-hsp70, heat shock 70 promoter; p-lef2, putative lef-2 promoter; p-polh, polyhedrin promoter. Black boxes represent sequences homologous to the 5′ and 3′ ends of lef-2; the striped box represents the HA tag sequence.