FIG. 3.
Temporal expression of lef-2 transcripts in infected cells. Sf21 cells were infected with either vAcwt-hE or vAcΔlef2-Rep-hE at an MOI of 5. Total cellular RNA was extracted from infected cells harvested at 0, 4, 8, 12, 16, 24, 36, and 48 h postinfection. Five micrograms of total RNA from each sample was resolved on a 1% formaldehyde gel and stained with ethidium bromide for visualization of 18S rRNA to ensure loading of equal sample amounts. The samples were then transferred onto a Hybond-N+ membrane (Amersham), hybridized with DIG-labeled lef-2 antisense RNA probes, and analyzed with a DIG detection kit. Arrows indicate bands corresponding to lef-2-specific transcripts.