FIG. 4.

Growth of 16681 parent and variants lacking one or both E protein glycans in mammalian cells. Virion-associated RNA release from BHK cells electroporated with 16681 parent or loss-of-glycan variant RNA transcripts (1 μg genomic RNA) is shown. Supernatant samples were collected at the indicated time points, and virion-associated DENV-2 RNA was measured by quantitative real-time RT-PCR. Error bars indicate the SEM for two real-time PCR determinations for each sample shown. Representative data from 2 experiments are shown.