TABLE 2.
Pseudoreversions and suppressor mutations selected in the E protein of 2 strains of DENV-2 lacking the Asn 67-linked glycan
| Variant (bold) or mutant derived therefrom | Growth in mammalian cells | Nucleotide changea | Amino acid changeb |
|---|---|---|---|
| rPUO.T69A | Yes | A1141 → G | Thr69 → Ala |
| rPUO.T69A/N124S | Yes | A1141 → G | Thr69 → Ala |
| A1307 → G | Asn124 → Ser | ||
| rNGC.T69A | No | A1141 → G | Thr69 → Ala |
| rNGC.T69V | Yes | A1141 → G | Thr69 → Val |
| C1142 → T | silent | ||
| rNGC.T69A/K126T | Yes | A1141 → G | Thr69 → Ala |
| A1313 → C | Lys126 → Thr |
a
Numbering from the 5′-terminal nucleotide in the DENV-2 NGC genome (9).
b
Numbering from the N-terminal residue in the E protein.