FIG. 3.

Chk2 depletion abrogates histone deacetylation at the DS. (A and B) D98/HR1 cells were transfected with siControl or siChk2 RNA and then assayed by ChIP for binding of EBNA1, AcH3, TRF2, ORC2, or control IgG at EBV regions for the DS (A) or OriLyt (B). ChIP DNA was quantified by real-time PCR as a relative percentage of the input. (C) D98/HR1 cells were transfected with siChk2 (+) or siControl (−) and then assayed for HDAC2 abundance by Western blotting (input) or after immunoprecipitation with IgG control or anti-TRF2 antibody, as indicated. Control Western blots for TRF2 are indicated at the bottom. The asterisk indicates a cross-reacting IgG band from the immunoprecipitating antibody. (D) HDAC activity assay from total cell extracts derived from siControl- or siChk2-transfected D98/HR1 cells.