TABLE 7.
Peripheral and intrahepatic CD4+/CD8+ T cell responses in chimpanezee CH5300
| Wk | T-cell response (no. of IFN-γ-secreting CD4+/CD8+ T cells) after stimulation with peptide poola: |
Total no. of T cells |
Total no. of positive pools |
|||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Core/E1 |
E2 |
p7/NS2 |
NS3-1 |
NS3-2 |
NS4A/B |
NS5A |
NS5B-1 |
NS5B-2 |
||||||||||||||
| P | IH | P | IH | P | IH | P | IH | P | IH | P | IH | P | IH | P | IH | P | IH | P | IH | P | IH | |
| −1 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 0 | 0 | 0 | 0 |
| 1 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 0 | 0 | 0 | 0 |
| 2 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 0 | 0 | 0 | 0 |
| 3 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 0 | 0 | 0 | 0 |
| 4 | ND | ND | ND | 457 | ND | 200 | ND | 3,327 | ND | 4,193 | ND | 437 | ND | 163 | ND | 240 | ND | 2,757 | 0 | 11,773 | 0 | 8 |
| 6 | ND | 395 | ND | ND | ND | ND | ND | 435 | ND | 2,770 | ND | ND | ND | ND | ND | ND | ND | ND | 0 | 3,600 | 0 | 3 |
| 7 | ND | 245 | ND | ND | ND | 2,275 | 103 | 2,770 | 190 | 4,165 | ND | 395 | ND | 2,300 | ND | 1,745 | ND | 3,355 | 293 | 17,250 | 2 | 8 |
| 8 | ND | 1,510 | ND | 2,025 | ND | 1,220 | 105 | 2,710 | 72 | 3,255 | ND | ND | 50 | 2,435 | ND | ND | ND | 1,685 | 227 | 14,840 | 3 | 7 |
| 9 | ND | ND | ND | ND | ND | ND | ND | 1,360 | ND | 2,105 | ND | ND | ND | 3,420 | ND | 1,015 | ND | 5,225 | 0 | 13,125 | 0 | 5 |
| 10 | ND | 150 | ND | ND | ND | ND | ND | 1,390 | ND | ND | ND | ND | ND | 515 | ND | ND | ND | 4,000 | 0 | 6,055 | 0 | 4 |
| 11 | ND | 1,470 | ND | ND | ND | 2,345 | ND | 2,495 | 160 | 1,730 | ND | ND | ND | 1,115 | ND | 770 | ND | 4,270 | 160 | 14,195 | 1 | 7 |
| 12 | ND | 3,030 | ND | ND | ND | ND | ND | ND | 203 | 390 | ND | ND | ND | ND | ND | ND | ND | 780 | 203 | 4,200 | 1 | 3 |
| 13 | ND | ND | ND | ND | ND | 685 | ND | ND | 135 | 105 | ND | ND | ND | ND | ND | ND | ND | 1,080 | 135 | 1,870 | 1 | 3 |
| 14 | ND | ND | ND | ND | ND | 675 | ND | 2,585 | 365 | 495 | ND | ND | ND | 410 | ND | 1,830 | ND | 5,590 | 365 | 11,585 | 1 | 6 |
| 15 | ND | 1,320 | ND | 1,470 | ND | 785 | ND | 185 | 127 | 550 | ND | ND | ND | 160 | ND | ND | ND | 3,840 | 127 | 8,310 | 1 | 7 |
| 16 | ND | 625 | ND | ND | ND | 155 | ND | ND | ND | 330 | ND | 195 | ND | ND | ND | ND | ND | 4,660 | 0 | 5,965 | 0 | 5 |
| 17 | x | ND | x | ND | x | ND | x | ND | x | 1,425 | x | ND | x | 1,505 | x | ND | x | 930 | x | 3,860 | x | 3 |
| 18 | ND | 1,710 | ND | ND | ND | 240 | ND | 1,115 | ND | 220 | ND | ND | ND | 485 | ND | ND | ND | 140 | 0 | 3,910 | 0 | 6 |
| 20 | ND | 2,280 | ND | ND | ND | 290 | ND | 235 | ND | 1,290 | ND | ND | ND | 155 | ND | ND | ND | 335 | 0 | 4,585 | 0 | 6 |
| 22 | ND | 990 | ND | ND | ND | ND | ND | ND | ND | ND | ND | 140 | ND | ND | ND | ND | ND | 6,080 | 0 | 7,210 | 0 | 3 |
| 24 | ND | 4,020 | ND | ND | ND | ND | ND | ND | ND | 805 | ND | ND | ND | 4,875 | ND | ND | ND | 1,670 | 0 | 11,370 | 0 | 4 |
| 26 | ND | 2,895 | ND | ND | ND | ND | ND | 1,245 | ND | ND | ND | 280 | ND | 340 | ND | ND | ND | 1,725 | 0 | 6,485 | 0 | 5 |
| 28 | ND | 1,365 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 210 | ND | ND | ND | 185 | 0 | 1,760 | 0 | 3 |
| 30 | ND | 130 | ND | ND | ND | ND | ND | 280 | ND | 275 | ND | 230 | ND | 220 | ND | ND | ND | 1,820 | 0 | 2,955 | 0 | 6 |
| 32 | ND | 345 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 2,110 | 0 | 2,455 | 0 | 2 |
| Total no. of positive time points | 0 | 16 | 0 | 3 | 0 | 10 | 2 | 13 | 7 | 16 | 0 | 6 | 1 | 15 | 0 | 5 | 0 | 20 | ||||
a
The number of IFN-γ secreting CD4+/CD8+ T cells is given at each time point. The cells had been stimulated with peptide pools, reflecting the covered genome region in ELISPOT assays. The number of cells in the peripheral blood (P) and intrahepatic (IH) compartments are shown. ND, not detected; x, not done.