FIG. 5.

The poly(I:C)-induced increase in CD8α⁺ DCs may contribute to the rapid clearance of apoptotic cells. A total of 2 × 10⁷ CFSE-labeled wild-type splenocytes (Ly5.1) were adoptively transferred into congenic wild-type recipients (Ly5.2). Splenocytes were harvested at 0 (untreated), 3, 6, 9, and 12 h after poly(I:C) treatment. (A) The capacities of Ly5.2⁺ CD8α⁺ CD11c⁺ and Ly5.2⁺ CD8α⁻ CD11c⁺ DCs to take up Ly5.1⁺ CFSE⁺ cells were correlated with CD40 expression at indicated the time points Doublets were excluded via pulse width, and gates were set upon Ly5.2⁺ CD11c⁺ 7-AAD⁻ cells. (B) The plot shows the percentages of host (Ly5.2) CD8α⁺ and CD8α⁻ CFSE⁺ DC populations following poly(I:C) treatment. Each data point is the average for three mice, and the data are representative of three independent experiments, each with three mice per data point. Error bars indicate standard deviations..