FIG. 7.

The LCMV-induced apoptosis of CD8β⁺ CD44^high T cells is partially dependent on Bim. C57BL/6 (wild-type) and Bim-deficient (Bim KO) mice were infected with LCMV-Armstrong i.p. Splenocytes were harvested at day 0 (untreated) and 3 days postinfection. DNA fragmentation was assessed via TUNEL staining after a brief in vitro culture of splenocytes for 5 h at 37°C (see Materials and Methods). (A) The plots show the percentages of untreated and LCMV-infected wild-type and Bim KO CD8β⁺ CD44^high T cells. (B) The plots show the percentages of untreated and LCMV-infected wild-type and Bim KO TUNEL⁺ CD8β⁺ CD44^high T cells. Each plot is representative of three mice per group, while the percentages are the averages for three mice per group. The data are representative of three independent experiments..