TABLE 1.
Comparative amino acid analysis of rVBS, HK116, and rMLV envelope proteinsa
| ORF | Protein (length [amino acids]) | Position | Amino acid in: |
||
|---|---|---|---|---|---|
| rVBS | HK116 | rMLV | |||
| 2a (9751-9954) | E (67) | ||||
| 2b (9824-10507) | GP2 (227) | 62 | Tyr | Tyr | His |
| 92 | Ile | Thr | Thr | ||
| 158 | Gly | Gly | Glu | ||
| 223 | Arg | Pro | Pro | ||
| 3 (10306-10797) | GP3 (rVBS and HK116, 163; rMLV, 168) | 80 | Leu | Leu | Val |
| 123 | Leu | Leu | Ser | ||
| 160 | Cys | Cys | Tyr | ||
| 164 | Stop | Stop | Gln | ||
| 165 | Phe | ||||
| 166 | Tyr | ||||
| 167 | Leu | ||||
| 168 | His | ||||
| 168 | Stop | ||||
| 4 (10700-11158) | GP4 (152) | 4 | Tyr | Tyr | Ser |
| 8 | Leu | Ser | Ser | ||
| 29 | Ala | Ala | Thr | ||
| 37 | Ile | Ile | Thr | ||
| 5 (11146-11913) | GP5 (255) | 69 | Leu | Leu | Pro |
| 72 | Gln | Gln | Lys | ||
| 81 | Asn | Asp | Asp | ||
| 100 | Ser | Gly | Gly | ||
| 104 | Asn | Gly | Gly | ||
| 106 | Met | Met | Val | ||
| 170 | Ala | Ala | Ser | ||
| 214 | Gly | Gly | Glu | ||
| 6 (11901-12389) | M (162) | 38 | Leu | Leu | Ser |
| 49 | Phe | Phe | Leu | ||
| 71 | Val | Val | Ala | ||
| 81 | Met | Thr | Thr | ||
| 122 | Ile | Val | Val | ||
| 150 | Phe | Phe | Cys | ||
| 154 | Ala | Thr | Met | ||
a
Amino acid substitutions located in the predicted ectodomain of each protein are in bold. All potential N-glycosylation sites are conserved in GP2 (amino acid 155), GP3 (amino acids 28, 29, 49, 96, 106, and 118), and GP4 (amino acids 33, 55, and 90).