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. 2010 Mar 3;84(10):4923–4935. doi: 10.1128/JVI.00206-10

TABLE 1.

Secondary structure estimates for gB(801-904) obtained from θ222 valuesa

Additive θ222, ° cm2 dmol−1 % Helicityb Absolute increase in helicity (%)
Protein alone (control) −6,916.3 26.0
0.01% SDS −11,281.5 36.4 10.4
0.05% SDS −9,312.5 31.7 5.7
0.5% Tween 20 −6,921.8 26.0 None
30% TFE −19,482.3 55.9 29.9
50% EtOH −17,540.1 51.3 25.3
a

Corresponding CD spectra are shown in Fig. 3.

b

Calculated using the formula [(θ222 − θ2220)/(θ222H − θ2220)] × 100, where θ222 = −38,000° cm2 dmol−1 and θ222 = 4,000° cm2 dmol−1 (32).