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Proviral copy analysis, based on real-time PCR analysis of proviral copy number in PBMC and in tissue-derived mononuclear leukocytes isolated from spleen, MLN, and IEL. HTLV-1 proviral copy number is expressed as the number of infected cells per 1 × 104 PBMC and was calculated as follows: {(HTLV-1 pol copy number)/[(rabbit GAPDH copy number)/2]} × 10,000.
