FIG. 4.

vICA-deficient viruses do not replicate within monocytes infected at early times of macrophage differentiation. (A and B) Mean viral titers recovered from THP-1 cells during a time course analysis. The results shown were obtained from an experiment done in parallel. (C) Viral titers recovered from cultures infected in duplicate for 7 days in the presence or absence of the viral DNA synthesis inhibitor PFA. Shown are the average values from an experiment that has been repeated. (D) IE⁺ cells in cultures infected with vICA-deficient viruses ΔUL36 and RC2792 expressed as the percentage of IE⁺ cells in cultures infected with the control viruses Towne-BAC and RQ2792, respectively. (E to H) Images of GFP⁺ fluorescence from infected THP-1 cells. Original magnification, ×100. For all experiments, an MOI of 3 was used following treatment with TPA and HC for 24 h.