FIG. 2.

The recruitment and activation of innate immune cells is altered by viral cytokine expression. Four-day-old BALB/c mice were infected with 8 × 10⁴ FFU of RSV/wt, RSV/IFN-γ, or RSV/IL-4 i.n. or left naïve. (A and B) Cells were taken from lungs, and total lung cell numbers after homogenization (A) and percentages of lymphocytes (B) were determined on days 4, 7, and 14 postinfection. (C to F) Lung macrophages (CD11c⁺ CD11b⁻ cells) were characterized by flow cytometry, measuring numbers (C) and MHCII (D), CD80 (E), and CD86 (F) expression levels. (G and H) Total numbers of NK cells (CD3⁻ DX5⁺) (G) and activation of NK cells/CD69⁺ (H) were also measured. Data are representative of two experiments, and points represent data for five mice per group ± SEM.