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. 2010 Mar 3;84(10):5158–5170. doi: 10.1128/JVI.00090-10

FIG. 6.

FIG. 6.

The first 105 aa of UL84 are sufficient for enhancement of expression governed by the IE2 proteins but not for susceptibility to proteasomal degradation. (A) The first 105 aa were added to the amino terminus of another viral protein, UL44 (Hyb). Coexpression of this protein with IE2 86 and IE2 40 was assessed, as was the expression of UL44. The expression of the Hyb protein was assayed using an antibody directed against UL84 and UL44. (B) Proteasome treatment was conducted as in Fig. 5, except that amount of Hyb protein was analyzed in combination with IE2 86 (86) and IE2 40 (40). Cells were either treated with the proteasome inhibitor MG132 (+) or mock treated with DMSO (−). UL84 and UL44 protein expression was assayed as a control. Actin serves as a loading control in both panels A and B. (C) RNA was analyzed for IE2 86 (86), IE2 40 (40), UL44 (44), and Hyb samples. All samples were normalized to G6PD. Values are shown as relative amplification (Amp), with the first sample in the graph having a value set to 1.