FIG. 3.

Viral cDNA synthesis of gradient fractions from WT and IN mutant viruses. Fraction samples from WT (A) and both IN mutant viruses, NL-C130S (B) and NL-ΔIN (C), were subjected to an endogenous reverse-transcription assay. After 16 h, each sample was analyzed for the early reverse transcription product using real-time PCR with primers specific for −sssDNA (gray bars). As a control, efavirenz was added to the reaction mixture to a final concentration of 7.5 μM (black bars). The results are expressed as copy numbers per microliter for each fraction with the indicated densities and are representative of at least three independent experiments for each virus.