FIG. 5.

Decrease in CypA incorporation into IN mutant virus. WT and IN mutant viruses prepared by transient transfections of 293T cells were concentrated over a 20% sucrose cushion, and the viral titer was measured by p24 ELISA. Fifty nanograms of p24-equivalent concentrated virus was separated on a 10% SDS-polyacrylamide gel, and Western blot analysis was carried out using a polyclonal anti-CypA antibody to measure viral incorporation of CypA (top) and a monoclonal antibody to CA (bottom).