FIG. 6.

Expression pattern of proinflammatory genes during FV3 infection. RT-PCR of PLs (1 × 10⁶ cells) isolated from pooled samples of two to three uninfected and infected (1 × 10⁶ PFU FV3) animals at days 1, 2, 3, and 6 postinfection using primers specific for Xenopus TNF-α, arginase 1 (Arg-1), IL-1β, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a housekeeping gene control. Except for GAPDH (25 cycles), 30 cycles were used. No signal was detected with RT-negative controls.