TABLE 3.
Detection of viral DNA, viral transcription, and infectious particles in kidney and PL lysates of outbred frogs at different times following FV3 infection
| Sample source | Time point (dpi) | Detection of virus by the indicated methoda |
||
|---|---|---|---|---|
| PCRb | RT-PCRb | TCID50 (PFU/mg) | ||
| Kidney | 1 | ++ (3/3) | + (3/3) | 185 |
| 6 | ++ (6/6) | + (6/6) | 1,161 | |
| 9 | +/− (2/3) | +/− (2/3) | 320 | |
| 14 | +/− (1/3) | ND (0/3) | 270 | |
| PLs | 1 | ++ (3/3) | ++ (3/3) | 2,161 |
| 3 | + (3/3) | + (3/3) | +/−c | |
| 6 | + (3/3) | +/− (2/3) | ND | |
| 14 | + (3/3) | ND | ND | |
| 20 | + (2/3) | ND | ND | |
| 30 | ND (0/6) | ND (0/6) | ||
a
ND, not detected.
b
The numbers in parentheses indicate the number of individuals with detectable signals/the number of individuals tested. ++, high signal intensity; +, medium signal intensity; +/−, weak, barely detectable signal intensity.
c
Some virus-induced death was observed at low dilutions.