FIG. 2.

PARP1 depletion enhances OriP-dependent plasmid DNA replication. (A) 293 cells were cotransfected with either shRNA targeting PARP1 or a nontargeting control and with OriP-EBNA1 containing plasmid and subjected to Western blotting. (B) Cells were assayed for transient DNA replication at 72 h posttransfection using DpnI resistance assays. DpnI/BamHI-resistant DNA (top panel) was compared to the total recovered DNA linearized by BamHI (lower panel). The ratio of the DpnI/BamHI to BamHI DNA is indicated below as the percent wild-type control replication (% wt). Experiments are shown in duplicate, and the average of four independent replicates (data not shown) was quantified.