Skip to main content
. 2010 Mar 10;84(10):4988–4997. doi: 10.1128/JVI.02333-09

FIG. 4.

FIG. 4.

Increased EBNA1 binding and prereplication complex formation at OriP in PARP-depleted cells. (A) 293-ZKO cells were transfected with control or PARP1-targeting shRNA and assayed by Western blotting for depletion of PARP1 or EBNA1. (B) 293-ZKO cells transfected with control or shPARP1 expression vectors, as shown in panel A, were subjected to immunoprecipitation with anti-EBNA1 or control IgG. Immunoprecipitates were then analyzed by Western blotting with anti-PAR or anti-EBNA1. (C) ZKO-293 cells transfected with control or shPARP1 were assayed by ChIP with antibodies to EBNA1 or control IgG and analyzed by quantitative PCR at the DS and control OriLyt DNA regions. (D) The same experiment as in panel C, except that ChIP was performed with antibodies to MCM3, ORC2, or control IgG. α, anti.