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. 2010 Mar 10;84(10):5314–5328. doi: 10.1128/JVI.02329-09

FIG. 6.

FIG. 6.

Exposure to MVA-HIV inhibits HIV replication in DC and DC-T-cell cocultures. (A) Impact of MVA-HIV on HIV replication in DCs. DCs were mock treated or incubated with MVA-HIV (1 h, MOI = 1), washed, and loaded with HIVNL-AD8 (2 h, high and low doses of 100 and 1 ng of HIV Gag p24/ml/106 cells, respectively), and HIV replication was monitored. (B) Kinetics of HIV replication in DC culture supernatants, assessed by HIV Gag p24 ELISA. (C) Impact of MVA-HIV on HIV transfer. DCs were treated as in panel A (HIVNL-AD8, 2 h, 1 ng of HIV Gag p24/ml/106 cells) and cocultured with activated autologous CD4+ T cells (1 DC/1 T cell). (D) Kinetics of HIV replication in DC-T-cell culture supernatants, assessed by HIV Gag p24 ELISA. As controls, DCs were cultured separately (DC alone) and CD4+ T cells were directly infected with the same HIV dose (T alone), and HIV replication was monitored. For each panel, data are means ± SD for duplicates and are representative of at least 3 independent experiments. d, day.