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. 2010 Mar 10;84(10):5391–5403. doi: 10.1128/JVI.02221-09

FIG. 5.

FIG. 5.

In vitro FRAP experiments with NS1-deYFP constructs. (A) NS1-deYFP. (B) K406M-deYFP. (C) E445Q-deYFP. (D) E444Q-deYFP. (E) R508A-deYFP. (F) R510A-deYFP. Average recovery in buffer without ATP is shown in blue, that in buffer with 1 mM ATP is shown in red, that in buffer with 1 mM AMP-PNP is shown in black, and that in buffer with 1 mM ATP-γS is shown in purple (only in panels A and D). The y axis is normalized average fluorescence, and the x axis is the time after bleaching, in seconds. Error bars show standard errors (SE). Twenty or more cells were analyzed within each condition. (G) Inverted gray-scale images of NS1-deYFP recovery in ivFRAP experiments under different imaging conditions. The bleached area is highlighted with a black line. Bar, 5 μm.