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Inhibition of PKR activity enhances MVMp protein synthesis. Tet-Off MEFs expressing the vaccinia virus E3 protein (vvE3) were induced overnight by tetracycline withdrawal (ON) and infected with MVMp at an MOI of 10. At the indicated time points (hpi), the cells were harvested and analyzed by Western blotting with the indicated antibodies. Uninduced cells (24) were maintained with 1 μg/ml tetracycline during the experiment.
