TABLE 2.
Minisequencing primers for SBE 1 and SBE 2 used in this study
| Marker | No. of poly(dC) copies | Neutral sequence (5′→3′) | Target specific sequence (5′→3′) | Orientationa | Size (nt) | Concn (μM) |
|---|---|---|---|---|---|---|
| SBE 1 | ||||||
| hsp65631 | AAGTCTGACAA | GTCTCGACCTCCTTGGC | R | 28 | 0.35 | |
| katG463 | CGTGAAAGTCTGACAA | GCCTTAAGAGCCAGATCC | R | 34 | 0.15 | |
| gyrA95 | CCACGTCGTGAAAGTCTGACAA | ACGCGTCGATCTACGACA | F | 40 | 0.5 | |
| katG203 | ACTAGGTGCCACGTCGTGAAAGTCTGACAA | GCTCATCGCCGAGCCA | F | 46 | 0.14 | |
| 16S rRNA1429 | ACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | GGCCGGTACAAAGGGC | F | 55 | 0.25 | |
| gyrB(675) | 2 | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | TCTCATAAACCTGAGACCACTC | R | 64 | 0.14 |
| gyrB(756) | 13 | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | AAGACGGGGTCAACGGT | F | 70 | 0.4 |
| gyrB(1410) | 15 | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | GTTTGTGCAGAAGGTCTGTAA | F | 76 | 0.13 |
| SBE 2 | ||||||
| 1977 | GTGAAAGTCTGACAA | GACTGCCAACGACGAA | R | 31 | 0.3 | |
| 3352929 | TCGTGAAAGTCTGACAA | CTGACATTGGTGCACAAAAC | R | 37 | 0.2 | |
| 74092 | GGTGCCACGTCGTGAAAGTCTGACAA | GCGGTGAACCCGGTACT | R | 43 | 0.58 | |
| 105139 | ACTAGGTGCCACGTCGTGAAAGTCTGACAA | AGTTTCACGGTTATCAGCG | R | 49 | 0.58 | |
| 2460626 | ACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | GGACCGAGGGTCTGGC | F | 55 | 0.58 | |
| 232574 | 4 | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | ACAGGGCAATCACCTCG | F | 61 | 0.2 |
| 311613 | 11 | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | CCGTTCGTAGCCGCAG | F | 67 | 0.2 |
| 913274 | 16 | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA | TCGCTGGACCCAATCTC | F | 73 | 0.5 |
a
Orientation of primer according to the genomic sequence of M. tuberculosis H37Rv. R, reverse; F, forward.