Nucleotide sequence alignment of the target region for the HSL-PCR for 23 HPV types. The nucleotides identical to the top sequence (HPV3) are indicated as dots. Within the alpha genus the HPV types are ordered per species, with species 2 consisting of HPV types 3, 10, 28, 29, 77, and 94; species 4 consisting of HPV types 2, 2 variant, 27, and 57; and species 8 consisting of HPV types 7, 40, 43, and 91.