TABLE 4.

Mechanism of action and in vitro drug sensitivity assays

Drug	Chemical family	Mode of action	Incubation period (h)	Drug sensitivity test reliability
				[3H]HXa	SYBR green	pLDH	HRP2
Chloroquine	4-Aminoquinoleine	Interaction with heme	48	Yes	Yes	Yes	Yes
			72	Yes	Yes	Yes	Yes
Artesunate	Sesquiterpene lactone	Oxidative stress	48	Yes	Yes	Yes	Yes
			72	Yes	Yes	Yes	Yes
Atovaquone	Hydroxynaphtoquinone	Inhibition of orotate biosynthesis	48	Yes	Yes	No	Yes
			72	Yes	Yes	Yes	No
Pyrimethamine	Antifolic compound	Inhibition of pyrimidines synthesis	48	Yes	No	No	Yes
		Inhibition of DHFR	72	Yes	Yes	Yes	Yes
T3/SAR97276	Bis-thiazolium	Inhibition of PC biosynthesis	48	Yes	No	No	Yes
			72	Yes	Yes	No	No
Doxycycline	Antibiotic	Inhibition of protein synthesis of apicoplast	48	Yes	No	No	Yes
			72	Yes	No	No	No
			96	Yes	Yes	No	No

^aHX, hypoxanthine.