TABLE 5.
Result of virus neutralization and HI tests using guinea pig antisera against BToV strain Aichi/2004 and the four newly isolated strains
| Test and strain | Antibody titer using guinea pig antiserum (R%)a |
||||
|---|---|---|---|---|---|
| Miyagi-2006TI/E | Gifu-2007TI/E | Hokkaido-2008TI/E | Gifu-2009TI/E | Aichi/2004 | |
| Neutralization test | |||||
| Miyagi-2006TI/E | 51,200 (100) | 10,240 | 40,960 | 20,480 | 12,800 |
| Gifu-2007TI/E | 32,000 (25.0) | 102,400 (100) | 32,000 | 12,800 | 8,000 |
| Hokkaido-2008TI/E | 12,800 (63.2) | 6,400 (28.0) | 25,600 (100) | 12,800 | 6,400 |
| Gifu-2009TI/E | 25,600 (70.7) | 6,400 (19.8) | 40,960 (100) | 20,480 (100) | 6,400 |
| Aichi/2004 | 20,480 (56.6) | 12,800 (31.3) | 20,480 (70.7) | 12,800 (62.5) | 10,240 (100) |
| HI test | |||||
| Miyagi-2006TI/E | 20,480 (100) | 6,400 | 32,000 | 12,800 | 6,400 |
| Gifu-2007TI/E | 640 (14.0) | 10,240 (100) | 800 | 400 | 400 |
| Hokkaido-2008TI/E | 25,600 (88.4) | 20,480 (17.7) | 51,200 (100) | 12,800 | 8,000 |
| Gifu-2009TI/E | 20,480 (69.9) | 12,800 (17.7) | 51,200 (89.4) | 16,000 (100) | 6,400 |
| Aichi/2004 | 20,480 (44.2) | 12,800 (19.8) | 32,000 (62.5) | 12,800 (63.2) | 12,800 (100) |
a
For the neutralization test results, the neutralization antibody titers are indicated, and percent antigenic-relatedness values (R%) are indicated in parentheses. For the HI test, HI antibody titers are indicated, and percent antigenic relatedness values (R) are indicated in parentheses. Identity values are indicated in boldface.