Skip to main content
. 2010 Mar 17;48(5):1939–1942. doi: 10.1128/JCM.02261-09

FIG. 1.

FIG. 1.

A schematic representation of the map location of primers used in this study, against a GenBank reference B. petrii 23S rRNA (accession no. AM902716). Primer WSbugF (nt 572 to 591) was designed based on the unique 23S rRNA gene identified previously by subtractive hybridization and amplifies a 1.9-kb region with primer 2490r (nt 2470 to 2488). The typing primer 23S F2 (5′ GGA ACT CGG CAA ATT GAC) binds to the conserved sequence from nt 1657 to nt 1674 and amplifies the variable region from nt 1690 to nt 1810 (in gray). This region contains the 78-bp diagnostic region, nt 1721 to nt 1798.