Fig. 1.

Overview and phylogeny of the Dictyostelium Roco protein family. (A) Domain organization of the Dictyostelium Roco family. The proteins are characterized by a conserved core that consists of a Roc, COR, and kinase domain, in addition to multiple regulatory domains. Red bars represent places where the genes were disrupted. (B) Phylogenetic tree of the Roc-COR-kinase domain modules of Roco proteins from Dictyostelium discoideum (Dd), Dictyostelium purpureum (Dp), Dictyostelium fasciculatum (Df), and Polysphondylium pallidum (Pp); human (Hs) LRRK2 was used as an outgroup. The tree was constructed with Mega 4.1 software, using an alignment that was created in ClustalW. Bootstrap values of >90 are indicated in the figure. Locus tags for all sequences are listed in Table S7 in the supplemental material. These sequence data were produced by the U.S. Department of Energy Joint Genome Institute (http://www.jgi.doe.gov/) and the Jena Centre for Bioinformatics (http://sacgb.fli-leibniz.de/cgi/index.pl), in collaboration with the user community.