Fig. 3.

Phenotypes of qkgA-null, roco11-null, and pats1-null cells. (A) roco11-null cells produce fruiting bodies with longer stalks than those of wild-type cells. AX2, roco11-null cells, and roco11-null cells expressing Roco11 from an extrachromosomal plasmid were starved on nutrient-free agar and allowed to develop. After culmination, pieces of agar were excised and fruiting bodies were photographed from the side. (B) QkgA expression regulates cell proliferation. Doubling times were calculated from three independent growth curves; average values ± SD of results for exponentially growing cells are presented. Data were analyzed with paired Student's t test; *, significantly less than AX2 at P < 0.05; **, significantly more than AX2 at P < 0.05. (C) pats1-null cells have a cytokinesis defect on a solid support. AX2 and pats1-null cells were grown on plates and in shaking culture and photographed. (D) Quantification of the number of nuclei per cell. Cells were stained with DAPI, and the amount of nuclei per cell was counted. Data shown are from 616 AX2 and 335 pats1-null cells.