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. 2010 May;9(5):784–794. doi: 10.1128/EC.00336-09

Fig. 2.

Fig. 2.

P. falciparum PF10_0164 is expressed in all life cycle stages examined. (A) RT-PCR data showing expression of PF10_0164 in P. falciparum midgut sporozoites (mgSpz), salivary gland sporozoites (sgSpz), and mixed blood stages (mixed BS). Thirty-five cycles of amplification were performed from RNA treated with (+) or without (−) reverse transcriptase. Controls include HSP70 for blood stages and CSP for blood stages and sporozoites. (B) P. falciparum salivary gland sporozoite labeled with antibodies recognizing PF10_0164. The protein shows internal, granular staining similar to that seen for PyUIS4 in P. yoelii. Overlay image includes DAPI for labeling nuclei. Scale bar, 5 μm.