Table 2.
Plasmids used in this study
| Name | Description | Reference or source |
|---|---|---|
| pBluescript II KS | General cloning plasmid (bla, MCS)a | Stratagene |
| pJET1.2 | Positive selection vector, pUC19 derivative (bla, MCS in eco47IR) | Fermentas |
| pSK275 | ptrA resistance gene flanked by SfiI sites in pBluescript II KS (syn. pME3024) | 27 |
| pSK259 | Recyclable phleomycin resistance cassette flanked by loxP attachment sites (syn. pME2891) | 25 |
| pSK393 | Integrative plasmid for expression of A. fumigatusnsdD (pniaD::nsdD::his2AtptrA)b | 16 |
| pSK379 | Integrative A. fumigatus expression plasmid carrying pgpdA-his2At cassette and ptrA | This study |
| pSK245 | nsdD genomic locus (EcoRI/BamHI) from A. fumigatus D141 in pBluescript II KS | This study |
| pSK263 | nsdD::loxP-Phleor/tk-loxP replacement cassette | This study |
| pSK491 | mat1-1::ptrA deletion cassette | This study |
| pSK492 | mat1-2::ptrA deletion cassette | This study |
| pSK494 | Synthetic, codon-optimized gfp2-5 allele for expression in A. fumigatus | This study |
| pSK496 | Synthetic, codon-optimized mCherry allele for expression in A. fumigatus | This study |
| pSK505 | Integrative plasmid for expression of GFP-H2A (pgpdA::gfp2-5::his2A ptrA) | This study |
| pSK507 | Integrative plasmid for expression of mCherry-H2A (pgpdA::mCherry::his2A ptrA) | This study |
a
MCS, multiple cloning site.
b
p, promoter; t, terminator.