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Intracellular localization of GFP-tagged Akt1, Akt2, and Akt3-1 lacking PTS1 sequences. Strains were grown on PDA for 3 days. GFP-1, pYTGFPc transformant (A and B); GA1Δ-1, pGFP-AKT1Δ transformant (C and D); GA2Δ-1, pGFP-AKT2Δ transformant (E and F); GA3Δ-1, pGFP-AKT3Δ transformant (G and H); DIC, DIC images; GFP, GFP fluorescence images. Bars = 10 μm.
