Table 1.
Plasmid | Constructiona | Source |
---|---|---|
pSH75 | trpCp-hph-trpCt | 26 |
pII99 | trpCp-nptII-trpCt | 44 |
pSB116 | trpCp-bar-trpCt | This study |
pYTGFPc | trpCp-GFP-trpCt | 19 |
pYTGFP-N | trpCp-GFP-trpCt | 19 |
pGFP-AKT1 | trpCp-GFP-AKT1-trpCt | This study |
pGFP-AKT2 | trpCp-GFP-AKT2-trpCt | This study |
pGFP-AKT3 | trpCp-GFP-AKT3-1-trpCt | This study |
pGFP-AKT1Δ | trpCp-GFP-AKT1ΔPTS1-trpCt | This study |
pGFP-AKT2Δ | trpCp-GFP-AKT2ΔPTS1-trpCt | This study |
pGFP-AKT3Δ | trpCp-GFP-AKT3-1ΔPTS1-trpCt | This study |
pcAaPEX6 | Cosmid containing AaPEX6 | This study |
pDNR-PEX6 | Plasmid containing AaPEX6 (6.5 kb) | This study |
pGDPEX6n | 5′AaPEX6::trpCp-nptII-trpCt::3′AaPEX6 | This study |
trpCp and trpCt, A. nidulans trpC promoter and terminator, respectively (37); AKT1ΔPTS1, AKT2ΔPTS1, and AKT3-1ΔPTS1, lacking nine nucleotides encoding C-terminal PTS1-like tripeptides.