Table 1.
Plasmids used in this study
| Plasmid | Constructiona | Source |
|---|---|---|
| pSH75 | trpCp-hph-trpCt | 26 |
| pII99 | trpCp-nptII-trpCt | 44 |
| pSB116 | trpCp-bar-trpCt | This study |
| pYTGFPc | trpCp-GFP-trpCt | 19 |
| pYTGFP-N | trpCp-GFP-trpCt | 19 |
| pGFP-AKT1 | trpCp-GFP-AKT1-trpCt | This study |
| pGFP-AKT2 | trpCp-GFP-AKT2-trpCt | This study |
| pGFP-AKT3 | trpCp-GFP-AKT3-1-trpCt | This study |
| pGFP-AKT1Δ | trpCp-GFP-AKT1ΔPTS1-trpCt | This study |
| pGFP-AKT2Δ | trpCp-GFP-AKT2ΔPTS1-trpCt | This study |
| pGFP-AKT3Δ | trpCp-GFP-AKT3-1ΔPTS1-trpCt | This study |
| pcAaPEX6 | Cosmid containing AaPEX6 | This study |
| pDNR-PEX6 | Plasmid containing AaPEX6 (6.5 kb) | This study |
| pGDPEX6n | 5′AaPEX6::trpCp-nptII-trpCt::3′AaPEX6 | This study |
a
trpCp and trpCt, A. nidulans trpC promoter and terminator, respectively (37); AKT1ΔPTS1, AKT2ΔPTS1, and AKT3-1ΔPTS1, lacking nine nucleotides encoding C-terminal PTS1-like tripeptides.