Table 2.
AK-toxin I and EDA production and pathogenicity of ΔAaPEX6 mutant strains
| Strain | Production (μg/ml)a of: |
Pathogenicityb | |
|---|---|---|---|
| AK- toxin I | EDA | ||
| GA1-1 (GFP-AKT1) | 3.3 | 24.8 | + |
| GFP-AKT1/ΔAaPEX6 mutants | |||
| GDP6-1 | NDc | ND | − |
| GDP6-2 | ND | ND | − |
| GDP6-3 | ND | ND | − |
| GFP-AKT1/ΔAaPEX6/AaPEX6 mutants | |||
| GDP6c-1 | 2.9 | 25.6 | + |
| GDP6c-2 | 2.5 | 22.0 | + |
a
AK-toxin I and EDA in culture filtrates were analyzed by reverse-phase HPLC. Each value represents the average of two determinations.
b
+, pathogenic; −, nonpathogenic.
c
ND, not detected.