Fig. 8.
Effects of TbPLK depletion on the progression of T. brucei procyclic cells synchronized and released in late S phase. (A) Strain 29-13 cells harboring both pZJM-TbPLK RNAi and TbCPC1-EYFP constructs were treated with 1.0 μg/ml tetracycline for 24 h to induce TbPLK-RNAi. While the RNAi was continuing, the cells were further treated with 0.3 mM hydroxyurea (HU) for 16 h to become synchronized to the late S phase and released with continued TbPLK RNAi. Cell samples were collected hourly after the release and subjected to flow cytometry analysis for DNA content. (B) Analysis of the data from flow cytometry with ModFit LT V3.0 software for estimation of percentages of cells in G1, S1, and G2/M DNA phases.