Fig. 7.

Ca²⁺-induced translocation of URE3-BP and EhC2A to the plasma membrane of amebic trophozoites. E. histolytica trophozoites were incubated at 37°C for 1 h in TYI-S-33 medium with 5 mM EGTA (A) or 1 mM CaCl₂ and 1 μM A23187 (B). Fixed permeabilized cells were probed with rabbit anti-EhC2A polyclonal antibody, Alexa Fluor 488 (green) goat anti-rabbit IgG (EhC2A), mouse anti-URE3-BP MAb 4D6, and Alexa Fluor 546 (red) goat anti-mouse IgG (URE3-BP). Nuclei were stained blue with 4′,6-diamidino-2-phenylindole (DAPI). Merged images demonstrate colocalization of the two proteins.